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pclnrx retrovirus expression vector  (Novus Biologicals)


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    Novus Biologicals pclnrx retrovirus expression vector
    Pclnrx Retrovirus Expression Vector, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pclnrx+retrovirus+expression+vector/pCLNRX+Retrovirus+Expression+Vector/pmc03039877-115-25-28
    Average 90 stars, based on 2 article reviews
    pclnrx retrovirus expression vector - by Bioz Stars, 2026-10
    90/100 stars

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    Plasmid Preparation:

    Article Title: Carrier-based intralymphatic cisplatin chemotherapy for the treatment of metastatic squamous cell carcinoma of the head & neck
    Article Snippet: .. The pCLNRX-turbo635 plasmid was constructed by inserting a 715-bp AfeI/HindIII fragment of the pTurboFP635C (Evrogen Inc, Moscow, Russia) into a 7675-bp AleI/HindIII fragment of the pCLNRX retrovirus-expression vector (Imgenex, CA, USA). ..

    Article Title: Carrier-based intralymphatic cisplatin chemotherapy for the treatment of metastatic squamous cell carcinoma of the head & neck
    Article Snippet: .. Tumor model & in vivo imaging The pCLNRX-turbo635 plasmid was constructed by inserting a 715-bp AfeI/HindIII fragment of the pTurboFP635C (Evrogen Inc, Moscow, Russia) into a 7675-bp AleI/HindIII fragment of the pCLNRX retrovirus-expression vector (Imgenex, CA, USA). ..

    Construct:

    Article Title: Carrier-based intralymphatic cisplatin chemotherapy for the treatment of metastatic squamous cell carcinoma of the head & neck
    Article Snippet: .. The pCLNRX-turbo635 plasmid was constructed by inserting a 715-bp AfeI/HindIII fragment of the pTurboFP635C (Evrogen Inc, Moscow, Russia) into a 7675-bp AleI/HindIII fragment of the pCLNRX retrovirus-expression vector (Imgenex, CA, USA). ..

    Article Title: Carrier-based intralymphatic cisplatin chemotherapy for the treatment of metastatic squamous cell carcinoma of the head & neck
    Article Snippet: .. Tumor model & in vivo imaging The pCLNRX-turbo635 plasmid was constructed by inserting a 715-bp AfeI/HindIII fragment of the pTurboFP635C (Evrogen Inc, Moscow, Russia) into a 7675-bp AleI/HindIII fragment of the pCLNRX retrovirus-expression vector (Imgenex, CA, USA). ..

    In Vivo Imaging:

    Article Title: Carrier-based intralymphatic cisplatin chemotherapy for the treatment of metastatic squamous cell carcinoma of the head & neck
    Article Snippet: .. Tumor model & in vivo imaging The pCLNRX-turbo635 plasmid was constructed by inserting a 715-bp AfeI/HindIII fragment of the pTurboFP635C (Evrogen Inc, Moscow, Russia) into a 7675-bp AleI/HindIII fragment of the pCLNRX retrovirus-expression vector (Imgenex, CA, USA). ..



    Similar Products

    pclnrx retrovirus expression vector  (Novus Biologicals)
    90
    Novus Biologicals pclnrx retrovirus expression vector
    Pclnrx Retrovirus Expression Vector, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pclnrx+retrovirus+expression+vector/pCLNRX+Retrovirus+Expression+Vector/pmc03039877-115-25-28
    Average 90 stars, based on 1 article reviews
    pclnrx retrovirus expression vector - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    pclnrx expression vector  (Novus Biologicals)
    90
    Novus Biologicals pclnrx expression vector
    Figure 1 Schematic illustrations of the plasmid constructs. The coding region of the anti-erbB2scFv was inserted into retroviral packaging vector pCL-Eco-DTM that lacked the transmembrane subunit of the envelope to generate pCL-EcoscFv. The p35 and p40 subunits of IL-12 as well as the sequence of the IRES were cloned into retroviral expression vector <t>pCLNRX</t> to yield pCL-IL12. The pCL-LacZ and pCL-Eco plasmids served as the controls. pCLMFG- LacZ, pCLNRX, and pCL-Eco were from the Moloney murine leukemia virus (MoMLV)-based pCL Vector System (RetroMax <t>System,</t> <t>Imgenex).</t> CMV, cytomegalovirus immediate-early promo- ter; RU5, direct repeat and unique sequence at 50 region of MoMLV RNA; c, packaging signal; LacZ, Escherichia coli b-galactosidase gene; LTR, long terminal repeat of MoMLV; NeoR, neomycin phosphotransferase gene; RSV, Rous sarcoma virus promoter; IL12p40, IL-12 p40 cDNA; IRES, encephalomyocarditis virus (EMCV) internal ribosome entry site; IL12p35, IL-12 p35 cDNA; SU, surface glycoprotein; TM, transmembrane; GAG, gag gene; POL, pol gene; SP, signal peptide; RBD, receptor-binding domain; PRR, proline-rich region; C, carboxyl terminal domain; VH, cDNA for variable fragment of heavy chain; VL, cDNA for variable fragment of light chain.
    Pclnrx Expression Vector, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pclnrx+retrovirus+expression+vector/pCLNRX+Retrovirus+Expression+Vector/pm19543243-30-19-22
    Average 90 stars, based on 1 article reviews
    pclnrx expression vector - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

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    Figure 1 Schematic illustrations of the plasmid constructs. The coding region of the anti-erbB2scFv was inserted into retroviral packaging vector pCL-Eco-DTM that lacked the transmembrane subunit of the envelope to generate pCL-EcoscFv. The p35 and p40 subunits of IL-12 as well as the sequence of the IRES were cloned into retroviral expression vector pCLNRX to yield pCL-IL12. The pCL-LacZ and pCL-Eco plasmids served as the controls. pCLMFG- LacZ, pCLNRX, and pCL-Eco were from the Moloney murine leukemia virus (MoMLV)-based pCL Vector System (RetroMax System, Imgenex). CMV, cytomegalovirus immediate-early promo- ter; RU5, direct repeat and unique sequence at 50 region of MoMLV RNA; c, packaging signal; LacZ, Escherichia coli b-galactosidase gene; LTR, long terminal repeat of MoMLV; NeoR, neomycin phosphotransferase gene; RSV, Rous sarcoma virus promoter; IL12p40, IL-12 p40 cDNA; IRES, encephalomyocarditis virus (EMCV) internal ribosome entry site; IL12p35, IL-12 p35 cDNA; SU, surface glycoprotein; TM, transmembrane; GAG, gag gene; POL, pol gene; SP, signal peptide; RBD, receptor-binding domain; PRR, proline-rich region; C, carboxyl terminal domain; VH, cDNA for variable fragment of heavy chain; VL, cDNA for variable fragment of light chain.

    Journal: Cancer gene therapy

    Article Title: Enhancement of antitumor activity of gammaretrovirus carrying IL-12 gene through genetic modification of envelope targeting HER2 receptor: a promising strategy for bladder cancer therapy.

    doi: 10.1038/cgt.2009.41

    Figure Lengend Snippet: Figure 1 Schematic illustrations of the plasmid constructs. The coding region of the anti-erbB2scFv was inserted into retroviral packaging vector pCL-Eco-DTM that lacked the transmembrane subunit of the envelope to generate pCL-EcoscFv. The p35 and p40 subunits of IL-12 as well as the sequence of the IRES were cloned into retroviral expression vector pCLNRX to yield pCL-IL12. The pCL-LacZ and pCL-Eco plasmids served as the controls. pCLMFG- LacZ, pCLNRX, and pCL-Eco were from the Moloney murine leukemia virus (MoMLV)-based pCL Vector System (RetroMax System, Imgenex). CMV, cytomegalovirus immediate-early promo- ter; RU5, direct repeat and unique sequence at 50 region of MoMLV RNA; c, packaging signal; LacZ, Escherichia coli b-galactosidase gene; LTR, long terminal repeat of MoMLV; NeoR, neomycin phosphotransferase gene; RSV, Rous sarcoma virus promoter; IL12p40, IL-12 p40 cDNA; IRES, encephalomyocarditis virus (EMCV) internal ribosome entry site; IL12p35, IL-12 p35 cDNA; SU, surface glycoprotein; TM, transmembrane; GAG, gag gene; POL, pol gene; SP, signal peptide; RBD, receptor-binding domain; PRR, proline-rich region; C, carboxyl terminal domain; VH, cDNA for variable fragment of heavy chain; VL, cDNA for variable fragment of light chain.

    Article Snippet: The PCR products were individually cloned into the pGEM-T easy vector (Promega, Madison, WI), and then transferred into the pCLNRX expression vector (Imgenex), yielding pCL-IL12 plasmid (Figure 1).

    Techniques: Plasmid Preparation, Construct, Retroviral, Sequencing, Clone Assay, Expressing, Virus, Binding Assay